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human ifn λ3 duoset elisa kits  (R&D Systems)


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    R&D Systems human ifn λ3 duoset elisa kits
    Impact of reduced NS1 levels on IFN expression. A549 cells were infected with RSV-Mnull/G1, RSV-Mnull/F1, RSV-Mnull, and RSV-rWT, or left uninfected (mock). Infected cell supernatant was examined at 24 hpi. (A) mRNA levels. Total RNA was extracted from infected cells and real-time PCR was performed (primers listed in Materials and Methods). IFN levels were normalized to RSV N protein mRNA levels. Fold change is indicated on the Y-axis. (B) Cytokine ELISA. Supernatants were harvested from infected cells, and cytokine levels were determined using commercially available human IFN-β <t>or</t> <t>IFN-λ3</t> specific kits. Error bars are the standard deviation of the mean of triplicate samples. *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001.
    Human Ifn λ3 Duoset Elisa Kits, supplied by R&D Systems, used in various techniques. Bioz Stars score: 95/100, based on 89 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+ifn+%CE%BB3+duoset+elisa+kits/pmc13106312-109-16-21?v=R%26D+Systems
    Average 95 stars, based on 89 article reviews
    human ifn λ3 duoset elisa kits - by Bioz Stars, 2026-08
    95/100 stars

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    1) Product Images from "Next-generation single-cycle respiratory syncytial virus vaccines with increased type I interferon induction yield robust systemic and mucosal responses in mice"

    Article Title: Next-generation single-cycle respiratory syncytial virus vaccines with increased type I interferon induction yield robust systemic and mucosal responses in mice

    Journal: Frontiers in Immunology

    doi: 10.3389/fimmu.2026.1778423

    Impact of reduced NS1 levels on IFN expression. A549 cells were infected with RSV-Mnull/G1, RSV-Mnull/F1, RSV-Mnull, and RSV-rWT, or left uninfected (mock). Infected cell supernatant was examined at 24 hpi. (A) mRNA levels. Total RNA was extracted from infected cells and real-time PCR was performed (primers listed in Materials and Methods). IFN levels were normalized to RSV N protein mRNA levels. Fold change is indicated on the Y-axis. (B) Cytokine ELISA. Supernatants were harvested from infected cells, and cytokine levels were determined using commercially available human IFN-β or IFN-λ3 specific kits. Error bars are the standard deviation of the mean of triplicate samples. *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001.
    Figure Legend Snippet: Impact of reduced NS1 levels on IFN expression. A549 cells were infected with RSV-Mnull/G1, RSV-Mnull/F1, RSV-Mnull, and RSV-rWT, or left uninfected (mock). Infected cell supernatant was examined at 24 hpi. (A) mRNA levels. Total RNA was extracted from infected cells and real-time PCR was performed (primers listed in Materials and Methods). IFN levels were normalized to RSV N protein mRNA levels. Fold change is indicated on the Y-axis. (B) Cytokine ELISA. Supernatants were harvested from infected cells, and cytokine levels were determined using commercially available human IFN-β or IFN-λ3 specific kits. Error bars are the standard deviation of the mean of triplicate samples. *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001.

    Techniques Used: Expressing, Infection, Real-time Polymerase Chain Reaction, Enzyme-linked Immunosorbent Assay, Standard Deviation



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    human ifn λ3 duoset elisa kits  (R&D Systems)
    95
    R&D Systems human ifn λ3 duoset elisa kits
    Impact of reduced NS1 levels on IFN expression. A549 cells were infected with RSV-Mnull/G1, RSV-Mnull/F1, RSV-Mnull, and RSV-rWT, or left uninfected (mock). Infected cell supernatant was examined at 24 hpi. (A) mRNA levels. Total RNA was extracted from infected cells and real-time PCR was performed (primers listed in Materials and Methods). IFN levels were normalized to RSV N protein mRNA levels. Fold change is indicated on the Y-axis. (B) Cytokine ELISA. Supernatants were harvested from infected cells, and cytokine levels were determined using commercially available human IFN-β <t>or</t> <t>IFN-λ3</t> specific kits. Error bars are the standard deviation of the mean of triplicate samples. *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001.
    Human Ifn λ3 Duoset Elisa Kits, supplied by R&D Systems, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+ifn+%CE%BB3+duoset+elisa+kits/pmc13106312-109-16-21?v=R%26D+Systems
    Average 95 stars, based on 1 article reviews
    human ifn λ3 duoset elisa kits - by Bioz Stars, 2026-08
    95/100 stars
    		  Buy from Supplier

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    Impact of reduced NS1 levels on IFN expression. A549 cells were infected with RSV-Mnull/G1, RSV-Mnull/F1, RSV-Mnull, and RSV-rWT, or left uninfected (mock). Infected cell supernatant was examined at 24 hpi. (A) mRNA levels. Total RNA was extracted from infected cells and real-time PCR was performed (primers listed in Materials and Methods). IFN levels were normalized to RSV N protein mRNA levels. Fold change is indicated on the Y-axis. (B) Cytokine ELISA. Supernatants were harvested from infected cells, and cytokine levels were determined using commercially available human IFN-β or IFN-λ3 specific kits. Error bars are the standard deviation of the mean of triplicate samples. *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001.

    Journal: Frontiers in Immunology

    Article Title: Next-generation single-cycle respiratory syncytial virus vaccines with increased type I interferon induction yield robust systemic and mucosal responses in mice

    doi: 10.3389/fimmu.2026.1778423

    Figure Lengend Snippet: Impact of reduced NS1 levels on IFN expression. A549 cells were infected with RSV-Mnull/G1, RSV-Mnull/F1, RSV-Mnull, and RSV-rWT, or left uninfected (mock). Infected cell supernatant was examined at 24 hpi. (A) mRNA levels. Total RNA was extracted from infected cells and real-time PCR was performed (primers listed in Materials and Methods). IFN levels were normalized to RSV N protein mRNA levels. Fold change is indicated on the Y-axis. (B) Cytokine ELISA. Supernatants were harvested from infected cells, and cytokine levels were determined using commercially available human IFN-β or IFN-λ3 specific kits. Error bars are the standard deviation of the mean of triplicate samples. *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001.

    Article Snippet: The levels of IFN-β and IFN-λ3 in the supernatants were determined using the human IFN-β and human IFN-λ3 DuoSet ELISA kits (R&D Systems, MN, USA), following manufacturer’s instructions.

    Techniques: Expressing, Infection, Real-time Polymerase Chain Reaction, Enzyme-linked Immunosorbent Assay, Standard Deviation